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게시판 (13,048)
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월동 조건 및 건조 방법에 따른 CMV 내성 GM 및 non GM 고추의 발아율과 생장
2021-05-11
ABSTRACT The effects of wintering conditions and drying methods on the germination rate of CMV-resistant peppers 'H-15', non-GM parental line 'P-2377' and commercial varieties were investigated. In the first year (2012), 'H-15' and 'P-2377' were overwintered for 4 months on the ground (1m height) and underground (10, 20, 30cm depth). Overwintered pepper fruits sown with black PE mulching in early April, both varieties germinated regardless of wintering treatment method. 'H-15' was grown and fruited in all treatments, but 'P-2377' was only fruited in overwintering treatments 20 to 30 cm underground. In the second year (2013) three varieties 'H-15', 'P-2377' and 'Buja' were treated in the overwintering as in 2012, and then sown without mulching. Germinated was achieved, but the weed density (152.6 plants/m 2 , Beckmacia syzigachne 94.6%) was high, so the seedling growth was very poor and no fruiting. The effect of drying methods (2 weeks of sun drying; dry-heat treatments at 50, 60, 70°C during 48 hours) on the germination rate of 4 varieties ('H-15', 'P-2377', 'Shingang' and 'Manita') was investigated in 2013. In the case of seeding immediately after drying treatment, all four varieties germinated in 50°C drying treatment, 'H-15' and 'Shingang' did not germinated at 60°C, and all varieties did not germinated in 70°C treatment. After drying treatment, it was stored at room temperature for 1 year, and a germination test (2014) was conducted. As a result, germination rated was low, but the trend was similar to that in 2013. Even if the color of dried peppers gets worse, it is believed that drying at 70°C before distribution can completely eliminate the germination rate of GM seeds and prevent environmental pollution.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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하구역 생태계 보전 및 관리강화를 위한 조사사업 개선방안
2021-05-11
ABSTRACT Since 2004, the Ministry of Environment has been surveying 2∼3 estuaries every year to preserve the estuaries with high ecological value. However, it is insufficient to present the preservation logic for the estuaries with excellent ecosystems as well as foster curiosity about the current situation of the estuaries and environmental characteristics of Korea. Despite the increasing awareness of Korea's natural ecosystem conservation and management, the comprehensive and systematic information collection system for the estuaries was in a state of stagnation. In fact, it was evaluated that the achievement of the fundamental research objectives of supporting estuary ecosystem management policy and preserving estuaries was insufficient. Recently, the importance of ecosystem conservation and management is increasing. In the future, the importance of estuary ecosystem management will be more emphasized, and it will be necessary to present an active conservation strategy for the conservation of estuaries with excellent nature with solid plans for the restoration and management of damaged estuaries. This study attempted to propose a development plan by analyzing and evaluating the survey system for the estuary ecosystem. First, as part of the "Inland Wetland" scheme, it was proposed to systematically investigate estuaries by integrating them with other wetland research projects. Second, in order to overcome the limitations of the current survey system, it was proposed to introduce a new survey system involving semi-professionals and citizens. Based on the above proposal, it is strongly urged that preparing a thorough and systematic investigation project in connection with the characteristics of estuaries is established from now on. Although there may be difficulties in the process of making and implementing a plan, it will be an opportunity to prevent and preserve the natural ecosystem through a thorough and systematic preparation.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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Characterization and evaluation of response to heat and chilling stress in exotic weeds using chloro
2021-05-11
ABSTRACT The occurrence of exotic weeds and their influx into farmlands due to climate change poses many problems. Therefore, it is necessary to generate a prediction model for the occurrence pattern of these exotic weeds based on scientific evidence and devise prevention measures. The photosynthetic apparatus is known as the most temperaturesensitive component of a plant cell and its initial response to temperature stress is to inhibit the activation of photosystem II. This study investigated the potential of OJIP transients in assessing temperature stress in exotic weeds. The four exotic weeds cur rently flowing into Korean farmlands include Amaranthus spinosus, Conyza bonariensis, Crassocephalum crepidioides, and Amaranthus viridis. These weeds were treated at 5°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, and 40°C and the OJIP curves and JIP parameters were measured and analyzed. The results showed that heat and chilling stress affected the photosystem II (PSII) electron transport of A. spinosus, whereas C. crepidioides and A. viridis were more affected by high-temperature stress than by low-temperature stress. Lastly, C. bonariensis showed resistance to both high and low-temperature stress. The results of this study suggest that OJIP transients and JIP parameters can be used to analyze damage to the photosynthetic apparatus by temperature stress and that they can serve as sensitive indicators for the occurrence pattern of exotic weeds.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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가시나무 용기묘 생산을 위한 적정 시비 수준 구명
2021-05-11
Abstract 본 연구는 우리나라의 대표적인 난대 상록활엽수종 중의 하나인 가시나무의 고품질 용기묘 생산을 위한 적정 시비수준을 구명하고자 실시하였다. 용기는 임업시설양묘용 플라스틱 용기(350 ㎖/구)를 사용하였고, 시비처리는 무시비구와 함께 수용성 복합비료인 Multifeed 19 (N:P:K =19:19:19, v/v)를 1000 ㎎․L -1 , 2000 ㎎․L -1 , 3000 ㎎․L -1 로 조절하여 시비하였다. 실험결과, 수고와 근원경 생장은 시비수준이 높아짐에 따라 높은 생장값으로 나타났다. 건물생산량도 전체적으로 수고 및 근원경 생장 결과와 유사한 경향으로 나타났다. 뿌리발달은 2000 ㎎․L -1 시비구에서 가장 왕성하였다. 묘목의 품질을 나타내는 지수인 QI (Quality Index)도 2000 ㎎․L -1 시비구에서 가장 높았다. 연구결과를 종합하면, 가시나무 용기묘의 우량한 묘목생산을 위한 적정 시비수준은 2000 ㎎․L -1 시비구로 판단된다.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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Nucleoredoxin2 (NRX2) Promotes Jasmonate‑Mediated Trichome Formation in Arabidopsis
2021-05-11
Abstract Thioredoxin (Trx) proteins are essential for the maintenance of cellular redox balance through thiol/disulfide exchange modification. In Arabidopsis, the Trx superfamily consists of multiple protein isotypes distributed in most cellular compartments. Although the functions of chloroplastic and cytosolic Trxs have been investigated in plants, the physiological role of nuclear Trx proteins remains elusive. Nucleoredoxin (NRX) is a nuclear Trx first identified in eukaryotic organisms. Arabidopsis possesses two NRX genes (AtNRX1 and AtNRX2), and the function of AtNRX2 has not been elucidated to date. In this study, we characterized the function of AtNRX2 using the atnrx2 knockout mutant, based on its comparison with the atnrx1 mutant. In atnrx2 knockout mutant plants, trichome number was significantly reduced compared with the wild type (WT; Col-0) and the atnrx1 mutant. In response to JA induction of trichome, trichome formation was markedly diminished in the atnrx2 mutant. In addition, expression levels of genes involved in trichome formation were reduced in the atnrx2 mutant compared with the WT and atnrx1 mutant. Overall, our results suggest that AtNRX2 plays a physiological role in JA-mediated trichome formation in Arabidopsis.
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Development of Tagaturonate 3‑Epimerase into Tagatose 4‑Epimerase with a Biocatalytic Route from Fru
2021-05-11
ABSTRCAT D-Tagatose, a low-calorie functional sweetener, is biotransformed from lactose via galactose. The discovery of an enzyme with 4-epimerization activity toward D-fructose to produce D-tagatose has been a major goal in the sugar industry. However, no enzymes used for the direct production of D-tagatose from D-fructose have been identified until now. Here, we identified a tagaturonate 3-epimerase from Thermotoga petrophila with latent 4-epimerization activity toward D-fructose. We developed this enzyme into tagatose 4-epimerase by increasing the 4-epimerization activity toward D-fructose using structure-based engineering, including favorable interaction analysis and active-site modifications, and random mutagenesis, including saturation mutagenesis and DNA shuffling after error-prone PCR. The developed tagatose 4-epimerase, which showed 184-fold higher 4-epimerization activity toward D-fructose than that of tagaturonate 3-epimerase, produced 213 g/L D-tagatose using D-fructose in 2 h. Our results suggest that the tagatose 4-epimerase is an alternative enzyme for D-tagatose production via a different biocatalytic route.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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태기산(강원도)의 관속식물상
2021-05-11
Abstract Mt. Taegi (1,261.4 m) located in Gangwon-do, is a geographically important region for plant biodiversity. But the biodiversity of Mt. Taegi has been seriously damaged by the invasion of foreign plants, the development of forests and so on. We conducted 21 field surveys between April and August in 2008 and March and October in 2018 to investigate the vascular flora in Mt. Taegi. Total 662 taxa were identified including 96 families, 338 genera, 580 species, 4 subspecies, 66 varieties and 12 formas. Among them, 28 taxa were Korean endemic plants and 8 taxa were rare plants designated by the Korea Forest Services, and 11 taxa were listed on the Red list of endangered plants and 127 taxa were designated as the floristic regional indicator plants according to the Ministry of Environment. The alien plants were 45 taxa, including R. acetosella that is the ecosystem disturbing species.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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공동주택의 식재설계와 시공 일치를 위한 수목 수관폭 표준화
2021-05-11
Abstract Unexpected problems in the design process lead to inconsistencies in design and construction. The design and construction inconsistency is greatly increased due to the design that does not reflect the characteristics of the tree, and the construction that does not reflect the intention of the designer. Eventually detailed design drawings and construction completion drawings show a big difference. The purpose of this study is to suggest the size of the tree symbol reflecting the exact tree crown width for the major trees that used for landscaping planting construction. The size of the tree symbol to be reflected in the planting design is 21 times larger than that of Zelkova serrata , 23 times larger than that of Quercus palustris , 17 times larger than that of Metasequoia glyptostroboides , 22 times larger than that of Prunus yedoensis . Further research is needend on the low-frequency analysis tree that of Liriodendron tulipifera, Lagerstroemia indica, Ginkgo biloba, Chionanthus retusa, Aesculus turbinata . The standard of crown width can be used to calculate the green cover ratio of apartment complexes.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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Seventeen Unrecorded Species from Gayasan National Park in Korea
2021-05-11
ABSTRACT Macrofungi play important roles in forest ecology as wood decayers, symbionts, and pathogens of living trees. For the effective forest management, it is imperative to have a comprehensive overview of macrofungi diversity in specific areas. As a part of the National Institute of Biological Resources projects for discovering indigenous fungi in Korea, we collected macrofungi in Gayasan National Park from 2017 to 2018. These specimens were identified based on morphological characteristics and sequence analysis of internal transcribed spacer (ITS) or the nuclear large subunit rRNA (LSU) region. We discovered 17 macrofungi new to Korea: Butyrea japonica, Ceriporia nanlingensis, Coltricia weii, Coltriciella subglobosa, Crepidotus crocophyllus, Cylindrobasidium laeve, Fulvoderma scaurum, Laetiporus cremeiporus, Lentinellus castoreus, Leucogyrophana mollusca, Marasmius insolitus, Nidularia deformis, Phaeophlebiopsis peniophoroides, Phanerochaete angustocystidiata, Phlebiopsis pilatii, Postia coeruleivirens , and Tengioboletus fujianensis . We described their detailed morphological characteristics.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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Bakkenolides and Caffeoylquinic Acids from the Aerial Portion of Petasites japonicus and Their Bacte
2021-05-11
Abstract Petasites japonicus have been used since a long time in folk medicine to treat diseases including plague, pestilential fever, allergy, and inflammation in East Asia and European countries. Bioactive compounds that may prevent and treat infectious diseases are identified based on their ability to inhibit bacterial neuraminidase (NA). We aimed to isolate and identify bioactive compounds from leaves and stems of P. japonicas (PJA) and elucidate their mechanisms of NA inhibition. Key bioactive compounds of PJA responsible for NA inhibition were isolated using column chromatography, their chemical structures revealed using 1 H NMR, 13 C NMR, DEPT, and HMBC, and identified to be bakkenolide B (1), bakkenolide D (2), 1,5-di-O-caffeoylquinic acid (3), and 5-O-caffeoylquinic acid (4). Of these, 3 exhibited the most potent NA inhibitory activity (IC 50 = 2.3 ± 0.4 µM). Enzyme kinetic studies revealed that 3 and 4 were competitive inhibitors, whereas 2 exhibited non-competitive inhibition. Furthermore, a molecular docking simulation revealed the binding affinity of these compounds to NA and their mechanism of inhibition. Negative-binding energies indicated high proximity of these compounds to the active site and allosteric sites of NA. Therefore, PJA has the potential to be further developed as an antibacterial agent for use against diseases associated with NA.
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