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게시판 (13,048)
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Improved production of deglucosylated platycodin D from saponins from balloon flower leaf by a food-
2021-12-28
ABSTRACT Platycosides, saponins contained in balloon flower, which have been used as food health supplements for respiratory diseases, have diverse pharmacological effects. Platycosides exhibit better pharmacological activity by hydrolyzing their own sugars. However, to date, there have been no studies on the production of deglucosylated platycodin D suitable for food applications. In this study, Pluszyme 2000P, which was derived from Aspergillus niger, a food-grade microorganism, was used to completely convert platycoside E into deglucosylated platycodin D. For an efficient and economical production of deglucosylated platycodin D, the productivity was improved approximately 2.4 times by application of high hydrostatic pressure and the discarded balloon flower leaf was used as a substrate. As a result, deglucosylated platycodin D was produced with the highest concentration (3.49 mg/mL) and productivity (581.7 mg/L/h) reported so far. Our results contribute to functional saponin production and the related food industries.
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Diversity and species distribution of lichens in Gwangneung Forest
2022-12-23
Abstract: Gwangneung Forest is a forest area that has been in existence for more than 550 years with a long history of natural and artificial forests in harmony. Lichens are not only a single organism but also a group of basic organisms formed from a stable symbiotic association between a fungus and algae and/or cyanobacteria. They are closely associated with forests and have formed an axis through Gwangneung Forest. From 2016 to 2021, a survey was conducted to investigate the lichen flora distribution by species, focusing on the viewing area within the Korea National Arboretum and the undisclosed research area centered in the Suribong Peak area. As a result of collecting and identifying 246 specimens, 68 species of lichens were identified in 11 orders, 22 families, and 39 genera. The tree with the largest number of lichens was Koelreuteria paniculata, followed by Sophora japonica, Ginkgo biloba, and Malus sieboldii. In trees belonging to Aceraceae, crustose lichens rather than foliose lichens were mainly found. Through this survey, three unreported species were discovered: Lepraria cupressicola, Leprocaulon nicholsiae, and Graphis pinicola. Lepraria cupressicola and Leprocaulon nicholsiae were distributed throughout the arboretum viewing area and Graphis pinicola was identified only in one viewing area.
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삽목 번식시 차광정도, 삽목용토 및 생장조절제 처리가 꼬리풀의 발근에 미치는 영향
2023-01-04
Abstract : This study was conducted to develop a mass production for a commercial use by cutting of 4 kinds of V. glabrifolia Kitag., V. pusanensis Y.N.Lee, V. glabrifolia Kitag. × V. Spicata 'Alba' and V. spicata 'Ulster Blue Dwarf' × V. longifolia . Veronica L. Effects of shade, media and concentrations of plant growth regulators on the rooting of Veronica L. were examined. The rooting rate of V. glabrifolia Kitag. was higher as a 60% than the V. pusanensis Y.N.Lee as a 20% in the control of commercial media. In shading treatment, the rooting rate was highest at 30% or 60% shading, and the 30% shading was the best in number of root and root length, but the 90% shading was lower than no shading treatment. For cutting media, the rock wool and 100% perlite increased the rooting rate by more than 10% compared to commercial media, and increased the number of roots by 2 or 3 times. However the Cocopeat of media was lower of rooting rate, root number, and root length compared to another treatment. In the plant growth regulator treatment, the rate of rooting increased in all treatment compared to control, and was highest at IBA 1,000mg·L-1 as a 82.2% and NAA 200 or 400mg·L-1 as a 82.2% or 84.4% respectively, in the V. glabrifolia Kitag. × V. Spicata 'Alba'. However, the root number and root length was decreased as the concentration of growth regulators increased.
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자작나무 종자 저장기간 및 온도처리에 따른 발아 특성
2020-05-26
초록 본 연구는 우리나라의 주요 조림수종인 자작나무(Betulaplatyphylla var. japonica Hara.) 종자의 저장기간 및 온도처리에 따른 발아 특성을 구명하고자 수행되었다. 공시수종 종자는 각각 1996년, 2003 년, 2012년, 2013년, 2014년, 2015년, 2016년 채집된 자작나무 종자이며, 발아실험은 5℃, 10℃, 15℃, 20℃, 25℃, 30℃, 35℃의 온도 조건에서 실시하였다. 저장기간에 따른 발아율(GR)의 조사 결과 2012 년 이전에 채집된 종자에서는 발아가 전혀 되지 않았다. 저장기간별 발아율은 2015년에 채집된 종자가 모든 온도에서 가장 높았고, 저장기간이 길어질수록 낮은 것으로 조사되었다. 온도별 발아율의 경우 1 5℃ 이하의 온도 조건에서는 모든 종자에서 발아가 전혀 되지 않고, 30℃에서 가장 높은 발아율을 보였 다. 그 외의 발아특성은 저장기간이 짧은 종자이고 발아율이 높은 온도일수록 발아기(T 50)와 평균발아일 수(MGT)가 낮고 발아속도(GS)가 높은 경향을 보였다. 발아균일도(GU)는 상대적으로 고온인 35℃에서 높은 것으로 나타났다.
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Cloning and characterization of a-L-rhamnosidase from Chloroflexus aurantiacus
2020-05-26
ABSTRACT Objective This study was conducted to characterize recombinant a-L-rhamnosidase from Chloroflexus aurantiacus and apply the enzyme in the production of isoquercitrin from rutin. Results The a-L-rhamnosidase from C. aurantiacus was cloned and expressed in Escherichia coli BL21 and purified as a soluble enzyme. a-L-rhamnosidase purified from C. aurantiacus has a molecular mass of approximately 105 kDa and is predicted to exist as a homodimer with a native enzyme of 200 kDa. The purified enzyme exhibited the highest specific activity for rutin among the reported isoquercitrin producing a-L-rhamnosidases and was applied in the production of isoquercitrin from rutin. Under the optimised conditions of pH 6.0, 50 °C, 0.6 U mL -1 a-L-rhamnosidase, and 30 mM rutin, a-L-rhamnosidase from C. aurantiacus produced 30 mM isoquercitrin after 2 h with a 100% conversion yield and productivity of 15 mM h -1 . Conclusions We achieved a high productivity of isoquercitrin from rutin. Moreover, these results suggest that a-L-rhamnosidase from C. aurantiacus is an effective isoquercitrin producer.
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NaOCl 처리 농도 및 시간에 따른 희귀식물 자란(Bletilla striata Rchb.f.)의 종자 발아 및 활력 연구
2020-05-26
Abstract This study was conducted to examine the vitality of Bletilla striata Rchb.f. seed treated with different concentrations sodium hypochlorite (NaOCl) for different lengths of time. This study also examined the effect of NaOCl treatment times and concentrations on swelling formation and seed germination of B. striata seed. The non-treated B. striata seed had the highest survival rate (82.7%) Treatment with more than 1.5% NaOCl negatively affected the seed survival rate, as compared to concentrations of less than 1%. The swelling formation and seed germination percentages were highest (90.1% and 94.1%, respectively) when seeds were treated with 0.5% NaOCl for 20 min. These results can be used as important basic data for the growth and restoration of B. striata and further suggest the possibility of individual restoration in habitats.
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국립세종수목원 교육, 체험프로그램 운영을 위한 세종시 교사들의 요구도 조사
2020-05-26
요약 본 연구는 세종시 각 급 학교 교육자들을 대상으로 교육·체험프로그램의 참여형태, 방문목적, 프로그램 참여율 향상방안, 운영 및 진행방식 등을 조사하여 세종수목원 운영 프로그램 마련에 활용하고자 한다. 조사는 2019년 3월 한 달 간 세종시 교육청 홈페이지를 통해 각 급 학교 교육자 200명을 대상으로 설문을 실시하였으며, 설문의 완성도가 떨어지는 17부를 제외한 183부에 대하여 SPSS ver. 20.0 이용하여 분석하였다. 선다형 문항은 카이제곱(χ 2 ) 독립성 검증(chi-Square Independent Test)과 교차분석을 수행하였으며, 학생별 유의성은 일원배치 분산분석(oneway ANOVA)을 이용하여 분석하였다. 교육·체험프로그램 개발·운영에 대한 설문조사 분석결과, 각 급 학교는 교육·체험 프로그램을 위해 주로 교외에서 연간 2∼5회, 회당 평균 2.5시간을 할애하고 있으며, 선호하는 주제는 자연과 환경에서 47%로 가장 높게 나타났다. 수목원 방문목적은 유의수준 95%에서 2개 항목이 유의한 것으로 나타나 학년별로 유의미한 차이를 보였다. 운영 프로그램의 참여율 향상은 프로그램 개발, 강사진, 교통수단 순으로 높게 나타났으며, 각 요소에 대한 학년별 차이는 관찰되지 않았다. 운영방식 중 시기, 시간, 접수방식 순으로 봄 (56.3%), 2시간(48.6%), 사전예약제 (85.2%) 응답이 높게 나타났으며, 진행방식은 그룹 편성, 장소, 인원, 교수법 순으로 동일학년 편성(55.2%), 실내·외 강의 (54.6%), 20∼25명(29.7%), 체험형(84.7%) 응답이 높게 나타났다. 야간운 영은 참석자(47.0%)가 조사되어 운영의 필요성과 더불어 참석에 희망하지 않는 사람들은 안전성(57.1%)에 대한 우려를 보였다. 본 연구 결과는 세종수목원 프로그램의 기본방향을 수립하고 설정하는데 매우 중요한 기초 자료로 활용하고자 한다.
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구미시 가로수 식재현황에 따른 만족도 분석
2020-05-26
요약 본 연구는 구미시민들의 가로수에 대한 인식도와 선호도 등에 대한 연구결과를 중심으로 향후 구미시의 가로수 식재에 대한 개선방안 분석은 다음과 같다. 구미시 가로수 식재현황 분석 결과 구미시에 식재된 가로수는 은행나무를 비롯해 총 19종 38,910그루의 가로수가 식재되어 있으며, 은행나무 10,449그루 26.7%, 느티나무 9,360 그루 23.9%, 이팝나무 5,178그루 13.2%, 왕벚나무 4,990그루 12.7%, 배롱나무 4,222그루 10.8%, 순으로 조사되었다. 인지도는 공기정화 기능 39.7%, 도시 미관 30.5%, 계절감 15.3%, 그늘 제공 13.3% 순으로 조사되었다. 선호도는 전문가 의뢰가 58.1%, 지역주민은 34.3%로 나타났으며, 만족도는 녹지환경의 변화 그렇다 46.7%, 나빠졌다 2.2% 조사되었다. 가로수의 생장 방해 요소는 병충해 40.6%, 대기오염 30.0%, 영양부족 13.2% 순으로 조사되었다. 가로수의 식재 개선방안은 가지치기 29.7%, 중앙분리대 24%, 화단조성 20.7%, 가로수 식재 20%순으로 조사되었다.
HOME > 주요사업 > 산림생물 보전·활용 증진 > 기술개발 성과 > 학술논문
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Community structure of macromoths along altitudinal gradients in Korea
2021-12-28
ABSTRACT This study was aimed to (1) document the macromoth species richness and diversity along altitudinal gradients, (2) investigate the community structure along altitudinal gradients, and (3) analyze the similarity within each altitude using nonmetric multidimensional scaling (NMDS) ordination. In this study, macromoth community structures were investigated in Mt. Baegun, Korea, along the altitudinal gradients in 2012 and 2015. Diversity indexes and NMDS ordination were used for community structure in each altitudinal gradient. In NMDS ordination, the result of altitude was more distinct than that of month. The results suggest that the altitudinal habitat might be more affected in species composition rather than in seasonal temperature.
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Antioxidant Activity, Total Phenolic Content, Total Flavonoid Content, and Total Anthocyanin Content of Vaccinium oldhamii Miq. Collected from 11 Regions of South Korea
2024-09-20
Abstract : We studied antioxidant activities of Vaccinium oldhamii Miq. from 11 regions in South Korea and blueberries, domestically produced and imported. Correlation analysis between V. oldhamii habitats, environmental factors, and antioxidant properties was conducted. DPPH RC50 values ranged from 220.44 to 902.38㎍/mL, ABTS from 524.29 to 1230.97 ㎍/mL, and FRAP from 1783.71 to 2235.78 ㎛ Fe (II)/g. V. oldhamii from Gumi showed highest DPPH activity, Taean and Haenam for ABTS, and Gimcheon for FRAP. V. oldhamii exhibited superior antioxidant activities compared to blueberries. Meteorological conditions correlated positively with ABTS and DPPH activities, negatively with wind speed and humidity affecting DPPH and phenolic, flavonoid, and anthocyanin contents. Based on these findings, it is suggested that V. oldhamii fruits collected from Gimcheon and Gumi regions can be effectively utilized as natural antioxidants derived from plant materials.
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이 누리집은 산림청 산하 공공기관 누리집입니다.
